study of correlation between prostate specific antigen and matrix metalloproteinase-2 activity in benign and malignant prostate hyperplasia

Authors

نسترن علی زاده

nastaran alizadeh امید صفا

omid safa محمدرضا خرمی زاده

mohammad reza khoramizadeh محمد پزشکی

mohammad pezeshki

abstract

there are emerging data on novel tumor markers such as matrix metalloproteinase-2 (mmp-2 or gelatinase-a), which play a key role in tissue invasion and metastasis. we designed an investigation to assess the usefulness of mmp-2 activity as compared to prostate specific antigen (psa) in cancer staging process. we have analyzed the circulating form of mmp-2 in serum samples of patients suffering from either benign prostate hyperplasia (bph, n = 54) or prostate cancer (pc, n = 26), and prostatitis (n = 4) as compared to control normal individual (n = 26), respectively. protein-content adjusted samples were separated by gelatin-embedded polyacrylamide gel electrophoresis then were subjected to densitometric analysis. total psa (tpsa) and free psa (fpsa) were quantified using a standard elisa technique. correlation coefficient (r) between tpsa and mmp-2 activity in patient group was +0.938 and in controls group was 0.799 ( p <0.01). in addition, (r) between tpsa and mmp-2 activity in pc patients was 0.940 and in bph patients was 0.962 ( p <0.01). (r) between fpsa and mmp2 activity in pc patients was 0.913, in bph patients was 0.644, and in prostatitis patients was -0.994 (p <0.01). these results demonstrate that mmp-2, compared to psa, might be considered as a better tumor marker in monitoring and screening patients with pc.

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Journal title:
iranian biomedical journal

جلد ۱۰، شماره ۱، صفحات ۲۷-۳۲

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